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60-mer oligonucleotide microarray experiment  (Agilent technologies)


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    Agilent technologies 60-mer oligonucleotide microarray experiment
    60 Mer Oligonucleotide Microarray Experiment, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligonucleotide+microarray+experiments/pmc03428664-190-21-20
    Average 90 stars, based on 1 article reviews
    60-mer oligonucleotide microarray experiment - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Exploring by whole exome sequencing patients with initial diagnosis of Rubinstein–Taybi syndrome: the interconnections of epigenetic machinery disorders
    Article Snippet: High-resolution array CGH Array CGH experiments were performed with a commercially available 400 K 60-mer oligonucleotide microarray slide (Agilent Technologies Inc., Santa Clara, CA, USA) in accordance with the manufacturer’s instructions.

    Article Title: Inactivation of Genes Encoding MutL and MutS Proteins Influences Adhesion and Biofilm Formation by Neisseria gonorrhoeae
    Article Snippet: Briefly, for the microarray experiments, an Agilent custom-designated 60-mer oligonucleotide array (Agilent-034141) was used.

    Article Title: Toxoplasma gondii Rhoptry Protein ROP16 Mediates Partially SH-SY5Y Cells Apoptosis and Cell Cycle Arrest by Directing Ser15/37 Phosphorylation of p53
    Article Snippet: Microarray experiments were performed by using Agilent human Genome 8× 60K oligonucleotide arrays.

    Microarray:

    Article Title: Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture.
    Article Snippet: .. Microarray experiments were performed using an Agilent DNA Microarray Scanner G2565BA with Feature Extraction v. 9.5.3 (Agilent Technologies), according to the manufacturer’s protocol. ..

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells
    Article Snippet: .. The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 μm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions. .. The original microarray images were analyzed with the Image Analysis/Feature Extraction software G2567AA (Version A.7.5, Agilent Technologies) using default or standard miRNA microarray settings.

    Article Title: Molecular signature of hepatitis B virus regulation by interferon-γ in primary human hepatocytes.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Aim: A complete cure for chronic hepatitis B virus (HBV) infection requires eliminat ion of covalent ly closed circular DNA (cccDNA); however, this remains to be clinically achieved.

    Article Title: Administration of molecular hydrogen during pregnancy improves behavioral abnormalities of offspring in a maternal immune activation model
    Article Snippet: .. DNA microarray experiments were performed using an Agilent Expression Array Whole Mouse Genome oligo DNA microarray (Agilent, Santa Clara, CA) at Hokkaido System Science (Sapporo, Japan) with the microarray service certified by Agilent. ..

    Extraction:

    Article Title: Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture.
    Article Snippet: .. Microarray experiments were performed using an Agilent DNA Microarray Scanner G2565BA with Feature Extraction v. 9.5.3 (Agilent Technologies), according to the manufacturer’s protocol. ..

    Expressing:

    Article Title: Molecular signature of hepatitis B virus regulation by interferon-γ in primary human hepatocytes.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Aim: A complete cure for chronic hepatitis B virus (HBV) infection requires eliminat ion of covalent ly closed circular DNA (cccDNA); however, this remains to be clinically achieved.

    Article Title: Administration of molecular hydrogen during pregnancy improves behavioral abnormalities of offspring in a maternal immune activation model
    Article Snippet: .. DNA microarray experiments were performed using an Agilent Expression Array Whole Mouse Genome oligo DNA microarray (Agilent, Santa Clara, CA) at Hokkaido System Science (Sapporo, Japan) with the microarray service certified by Agilent. ..

    Hybridization:

    Article Title: Variable expressivity of syndromic BMP4-related eye, brain, and digital anomalies: A review of the literature and description of three new cases
    Article Snippet: The array was analyzed with the Agilent scanner using the Feature Extraction software (v9.1 Agilent Technologies). .. Array comparative genomic hybridization (aCGH) experiments were performed using Agilent Human Genome CGH 60K oligonucleotide arrays (Agilent, Santa Clara, CA, USA; www.agilent.com ) with the ISCA design ( www.iscaconsortium.org ). .. The arrays were analyzed with the Agilent scanner and the Feature Extraction software (v.9.1.3).



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    60-mer oligonucleotide microarray experiment  (Agilent technologies)
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    60 Mer Oligonucleotide Microarray Experiment, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from <t>microarray</t> analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .
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    Comparison of transcript quantification by Affymetrix <t>microarray</t> with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.
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    Comparison of transcript quantification by Affymetrix <t>microarray</t> with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.
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    A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Journal: Journal of Alzheimer's Disease Reports

    Article Title: Transcriptomic Analysis of Alzheimer’s Disease Pathways in a Pakistani Population 1

    doi: 10.3233/ADR-230146

    Figure Lengend Snippet: A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Article Snippet: The oligonucleotide microarray experiments were conducted by EpigenDx (Boston, MA) using the Affymetrix U133 Plus 2.0 Array platform, which has comprehensive coverage of the whole transcribed human genome on a single array.

    Techniques: Derivative Assay, Microarray, Expressing, Activation Assay

    Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Quantitative Protein and mRNA Profiling Shows Selective Post-Transcriptional Control of Protein Expression by Vasopressin in Kidney Cells *

    doi: 10.1074/mcp.M110.004036

    Figure Lengend Snippet: Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

    Article Snippet: To address whether changes in protein abundance measured by SILAC LC-MS/MS are generally because of corresponding changes in mRNA levels, we carried out oligonucleotide microarray experiments (Affymetrix) in mpkCCD cells treated in the same manner (0.1 n m dDAVP or vehicle for 5 days).

    Techniques: Microarray, Quantitative RT-PCR